Journal: Translational Vision Science & Technology

Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

doi: 10.1167/tvst.14.11.21

Figure Lengend Snippet: Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC 35984), a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of HCEC viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.

Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

Techniques: Fluorescence, Microscopy, Staining, Calcein AM Assay

Journal: Translational Vision Science & Technology

Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

doi: 10.1167/tvst.14.11.21

Figure Lengend Snippet: HCEC viability after exposure to 17 S. epidermidis strains at varying concentrations. ( A ) Nonpathogenic strains. ( B, C ) Pathogenic strains. Asterisks indicate a significant decrease in cell viability compared with the PBS control. * P < 0.05; ** P < 0.01; *** P < 0.001; *** P < 0.0001.

Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

Techniques: Control

Journal: Translational Vision Science & Technology

Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

doi: 10.1167/tvst.14.11.21

Figure Lengend Snippet: HCEC viability distinguished pathogenic from nonpathogenic S. epidermidis strains. ( A ) Heatmap illustrating HCEC viability following exposure to varying concentrations of 12 pathogenic and 5 nonpathogenic strains. Comparison of HCEC viability after exposure to ( B ) 104, ( C ) 105, ( D ) 106, ( E ) 107, and ( F ) 108 CFU/mL of pathogenic and nonpathogenic groups. ROC analysis of the diagnostic performance of HCEC viability at ( G ) 104, ( H ) 105, ( I ) 106, ( J ) 107, and ( K ) 108 CFU/mL in distinguishing the pathogenic S. epidermidis strains. ns, not significant. ** P < 0.01.

Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

Techniques: Comparison, Diagnostic Assay